cDNA

Gene Expression Changes after Heat Shock by cDNA Array Analysis

热休克诱导基因表达谱的变化

Results The gloverin cDNA sequence designed artificially was expressed in COS 7 cells.

结果　人工设计的葛佬素cDNA序列在COS7中得到表达.

The cDNA sequencing was performed to analyze the change of FV coding sequence by splice site mutation.

通过cDNA测序分析剪切位点突变引起编码序列的变化。

Gene expression profile of rat mesenchymal stem cell spontaneous transformation determined by cDNA microarray analysis

芯片分析自发恶性转化的大鼠骨髓间充质干细胞的基因表达谱

Objective: To clone latent infection membrane protein 2A ( LMP2A) cDNA of Epstein Barr virus and study its genetic engineering vaccine.

目的：克隆爱泼斯坦巴尔病毒（EBV）潜伏期膜蛋白2A（LMP2A）基因，进一步研究其基因工程疫苗。

Objective : To clone the hTPO cDNA and study its expression level in COS? 7 cells.

目的: 克隆人血小板生成素(hTPO)cDNA,并研究其在COS - 7细胞中的表达.

Use of Poliovirus cDNA 5 ′ NCR Together with Its Polymerase Gene Can Increase Foreign Gene Expression

利用脊髓灰质炎病毒5'NCR和RNA聚合酶基因可提高外源基因表达

A large full-length gene bank has been contructured through large-scale cDNA cloning and sequencing.

本实验室大规模的cDNA克隆与测序工作积累了大量的全长基因库.

The cDNA was subcloned into prokaryotic expression vector pET 3 d and overexpressed in E . coli BL 21 ( DE 3 ).

将该cDNA插入原核表达载体pET3d并在 大肠杆菌 BL21 ( DE3 ) 中过量表达.

Methods Total RNA was extracted from peripheral blood lymphocytes ( PBL) of patients with glioma and subjected to reverse transcription PCR. Variable region genes ( Vk and VH gene) were amplified from the cDNA by using nested polymerase chain reaction.

方法从脑胶质瘤患者外周血淋巴细胞（PBL）中提取细胞总RNA，经逆转录后用套式多聚酶链反应（PCR）分别扩增抗体轻链VK和重链VH基因。

CDNA cloning of a novel gene related to apoptosis by differential display RT PCR

用差别显示法寻找细胞凋亡的相关基因

Selective detection of hepatitis B virus mRNA by random amplification cDNA ends

乙型肝炎病毒mRNA的选择性检测

Conclusions The human leptin gene cDNA clone was constructed by RT PCR.

【结论】以逆转录聚合酶链反应方法成功地构建了人瘦素基因cDNA克隆。

Photobiotin-labelled hantavirus cDNA probe prepared from recombinant M segment of R22 strain

光敏生物素标记流行性出血热病毒R22株M片段cDNA探针的研究

CDNA-AFLP differential patterns for 5 selected genes were confirmed via real-time reverse transcription PCR analysis.

用荧光实时定量PCR分析法证实了cDNA-AFLP基因表达结果。

Clone of EPO Gene from Human Fetal Liver cDNA by Reverse Transcription and PCR

人胎肝cDNA中EPO基因的克隆

Objective To construct a cDNA expression library of Periplaneta Americana nymph.

目的　构建美洲大蠊若虫cDNA表达文库.

Each plasmid contains a cDNA insert that was cloned via EcoRI and XhoI.

每个质体含有一段cDNA,此插入基因是利用EcoRI与XhoI来 复殖的.

When GAM cDNA was transfected into COS cells, it appears as an 24 kDa expressing band.

将GAM分子cDNA转染COS细胞 后, 细胞裂解液电泳可见一分子量约为24×103的表达带,与预期的分子量相符合.

Expression of the Tea cystatin cDNA in E.coli

茶树巯基蛋白酶抑制剂基因cDNA在大肠杆菌中的表达

E . coli DH 5α was transformed by pUC 18 recombined with PRV CP gene cDNA.

香木瓜环斑病毒(PRV) 外壳蛋白(CP)基因cDNA克隆 到pUC18上构建成 大肠杆菌 (Escherickiacoli)表达载体.

CDNA Cloning, Sequencing and Expression of Human nov Gene

人nov基因全长cDNA克隆、测序及其组织表达谱分析

The GenBank accession number is AF 265294. The WVDE cDNA is 1, 746 bp long.

PCR法 得到小麦紫黄质脱环氧化酶(WVDE)的全系列,cDNA的 全长为1, 746.

A high throughput in silico data analysis system for cDNA library, CLASP, was constructed by bioinformatic approaches.

应用生物信息学方法, 构建了一套针对cDNA或 EST文库 的高通量、自动化分析体系, CLASP(cDNALibraryAnalysisSystemPrimary).

Comparison of the sensitivity between digoxigenin-and biotin-labelled cDNA probes

Dig和Biotin标记的流行性出血热病毒cDNA探针敏感性比较

The homologue of monkey ESc-615 was cloned in rat by screening rat epididymis cDNA library.

为得到该基因在大鼠中的同源基因,采用了筛选大鼠附睾cDNA文库的方法,得到了猕猴附睾 ESc -615在大鼠附睾中的同源基因.

Phylogenetic trees show that there are 64 MADS-box genes encoding different proteins, including 46 cDNA sequences.

结果表明,水稻中有64个MADS-box基因编码不同氨基酸, 其中已知cDNA序列的为43个.

Objective : To clone and sequence cDNA of human lymphotoxin ( LT ) .

目的: 克隆人 淋巴毒素cDNA并测序.

Cloning of human brevican cDNA and the expression of its mRNA in human Glioma.

人短蛋白聚糖cDNA的克隆及其mRNA在人脑胶质瘤中表达的研究.

Identification of a Rice cDNA Encoding the Acyl CoA binding Protein ( ACBP )

一种水稻酰基辅酶A结合蛋白cDNA的鉴定 ( 英文 )